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The study of molecular biology involves examining cellular constituents, such as DNA, RNA, proteins, and enzymes, elucidating their interactions. Techniques like PCR and gene sequencing are employed for genetic material analysis, revealing molecular mechanisms in processes like replication and transcription. Gene expression, the process translating genetic information into functional proteins, is tightly regulated, influencing cellular functions and responses. Understanding gene expression provides insights into disease mechanisms and guides biotechnological applications, offering fundamental insights into genetic information utilization in living organisms.